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College students Rich Stoker and Halley Smith were rock climbing deep in a remote forest of West Virginia. When Rich reaches the top, he prepares to help Halley up, but is suddenly murdered. Someone then begins to yank Halley up the cliff so she cuts the rope and falls to the ground before attempting to escape but before she rushes to the car, she trips over a piece of barbed wire and then gets pulled back into the woods, screaming.
Medical student Chris Flynn (Desmond Harrington) drives through the mountains of West Virginia on his way to a business meeting, but a chemical spill in the road ahead forces him to take a different route. After being distracted for a moment, he collides with another car that is stopped in the middle of the road since its tires have been punctured by a piece of barbed wire. The car belongs to a group of college students on a camping trip: Jessie (Eliza Dushku), Carly (Emmanuelle Chriqui), Scott (Jeremy Sisto), Evan (Kevin Zegers) and Francine (Lindy Booth).
Evan and Francine stay to watch the cars as the others go to find help. Soon after, both of them were murdered by unknown attackers when they investigate into the woods. Meanwhile, the others find an isolated cabin and go inside to use a phone. After looking around for a moment, they are horrified to find human body parts. They quickly attempt to escape but are instead forced to hide inside when they see the family returning home. Three disfigured cannibalistic mountain men "Three Finger" (Julian Richings), "Saw Tooth" (Garry Robbins) and "One Eye" (Ted Clark) enter the cabin, bringing Francine's dead body. The four silently watch in horror as her body is messily amputated and then eaten.
Hours later after the cannibals fall asleep, they quietly attempt to escape. However, Saw Tooth awakens, alerts the other cannibals, and begins to chase them through the forest. The group climb up to a nearby clearing where they find hundreds of cars that belonged to the family's previous victims. When the cannibals arrive, Chris distracts their attention so the others can run for help, but he is shot in the leg by Saw Tooth, leaving Scott to distract them instead, and he gets away. The girls help Chris into their truck and quickly drive down a nearby path to pick up Scott. They find him, but as he runs to the car, Saw Tooth kills him with arrows and takes his body back to their cabin.
Chris, Jessie and Carly reach a dead end and have to continue on foot. They stumble upon an old watchtower and find a radio inside, which they use to call for help, but do not get a response. Later, they see the cannibals armed with torches searching at the foot of the watchtower. The radio starts responding to their call, alerting the cannibals. Unable to get inside, the cannibals set the tower on fire to try and burn the group alive, but they escape by jumping out of the window into a nearby tree. As they hide, Three Finger catches Carly and decapitates her.
After escaping, Chris and Jessie hide in a cave near a waterfall until the next morning. Just as they find a road out of the woods, the mountain men find them, pushing Chris down the hill and taking Jessie back to their cabin. Chris survives the fall and meets a police officer, but before Chris is able to convince the police officer of what is happening, the man is shot in the eye with an arrow. Chris attempts to drive away in the police truck, but cannot find the key, so he instead hitches a ride underneath the truck as it is driven back to the cabin by Saw Tooth, where Jessie is tied to a bed and gagged. As she is about to be killed, Chris drives through the building and runs into One Eye. He then unties Jessie and they fight the cannibals together. They escape and Chris manages to kill all of the cannibals by blowing up their cabin with a shotgun. Chris and Jessie drive off the road with the cannibals' pickup truck.
The credits are interrupted by a scene showing a deputy sheriff investigating and picking over the remains of the destroyed cabin. Three Finger, who survived the explosion, rises to attack the deputy. His insane laughter is heard, as the credits continue to roll.